Ae (Aedes aegypti) / tobd

Treatment exposure modality:	Olfaction / Contact with choice
Efficacy type:	Repellency (%) Duration on warm body
Stock solution:	20 mM in EtOH
Volume / datapoint:	300 μL (triplicates)
Starting concentration:	1875 μmol/m²

In Brief:

The Invenesis mosquito adult repellent assay evaluates the effect of treated surfaces on the number of landing of adults Aedes aegypti and the total time spent by mosquitoes on a warm body.

Adult mosquitoes are offered a treated heated surface (warm body) and a nontreated warm body separated by defined distances. The number of landing and the time spent on each warm body is recorded automatically by machine vision. For each of the 2 warm bodies, repellency (based on the number of landings) is expressed in % of the negative controls, 100 % meaning no landings on the warm body. The time spent on each warm body is also expressed in % of the negative controls, 100 % meaning that the mosquitoes spent the same time on the warm body as in an experiment with the treated warm body having received a placebo treatment. This assay allows to estimate the distance of protection induced by a treated surface.

Definitions:

To ensure flawless communication, the following definition section introduces the vocabulary used at INVENesis. You will find this vocabulary on our quotes, raw data files, processed data files, preliminary and final reports. Open the Lexicon below.

Lexicon

Assay Preparation:

Test plate: microplate in which an assay is run
Mother plate: microplate containing stock solutions
Stock solution: solution contained in mother plates out of which dilutions will be made
Daugher plate: microplate containing solutions used to treat test plates, usually dilution series

Type of Assays:

Organism-based assays : functional assay measuring a phenotypic response on a whole organism. Typically, motility, development, deterrency, repellency.
Electrophysiology assays: functional assay measuring the electrical respons of a receptor or an organ to a stimulus
Stock solution: solution contained in mother plates out of which dilutions will be made
Daugher plate: microplate containing solutions used to treat test plates, usually dilution series

Treatment exposure modalities:

Treatment modalities relate to the way the treatment is applied. Different treatment modalities help define how an active ingredient is most susceptible to act in a field case. Some compounds are more active when eaten, others are more active when applied externally on an organism. The following vocabulary is used in the list of assays:

Tarsal:

Tarsal: individuals are allowed to walk on surfaces treated with a formulated active ingredient.
Tarsal with choice: only a proportion of the surface accessible to individuals is treated.
Tarsal without choice: the whole surface accessible to individuals is treated so that individuals are unable to rest on untreated surfaces.

Topical:

Topical: direct application of a known amount of formulated active ingredient on a single individual

Oral:

Oral: voluntary feeding on medium treated with a formulated active ingredient.
Oral with choice: both a treated and an untreated medium are provided.
Oral without choice: only one treated medium is provided.

Immersion:

Immersion: individuals are immersed in a treated formulation.
Permanent Immersion: individuals are immersed for the whole duration of the assay.
Temporary Immersion: the treatment is removed after temporary immersion of individuals.