Electrophysiology assays represent our second main domain of activity. The Xenopus oocyte expression system allows to express functional ion-channels in the membrane of individual cells and measure their physiological function.
We measure the effect of compounds on ligand-gated ion-channels, voltage-gated ion-channels, GPCRs and transporters. Oocytes are injected with cDNA or cRNA encoding the receptor of interest using an automated injection platform (Roboinject). The effect of compounds on the heterologously expressed channels are recorded using an automated two electrodes voltage-clamp automate (HiClamp, Multichannelsystem).